Within the scope of Aycan Kayrav's MSc thesis, the article “Revealing the role of the X25 domains through the characterization of truncated variants of amylopullulanase enzyme from Thermoanaerobacter brockii brockii” supervised by Prof. Dr. Nevin Gül Karagüler in the Department of Molecular Biology & Genetics was published in the International Journal of Biological Macromolecules.

Today, more than 3.000 enzymes are used in industrial fields. However, the usage of enzymes in the industry is limited because of the loss of the stability of enzymes under harsh industrial process conditions. The discovery of extremophilic organisms and their extremozymes, together with protein engineering studies, helps to overcome this problem. In our previous study, the amylopullulanase (apu) enzyme from the thermophilic, anaerobic bacterium Thermoanaerobacter brockii brockii (tbb), and its two truncated variants were recombinantly produced and characterized to be used in the starch industry (Mumcu et al. 2023). In this paper, for the first time, the role of the X25 domains of the tbbapu enzyme was revealed by the construction of four more truncated variants using protein engineering studies. Sequence alignment, characterization, and structural studies of these six truncated variants of the Tbbapu enzyme all prove that the X25 domains are a strong carbohydrate-binding domain candidate. It was also represented that, the X25 domains play an important role in the stability of the enzyme against thermal, detergent, inhibitors, and organic solvents in industrial applications. 

For further information:

Aycan Kayrav, Hande Mumcu, Naciye Durmus, Nevin Gul Karaguler (2024) “Revealing the role of the X25 domains through the characterization of truncated variants of amylopullulanase enzyme from Thermoanaerobacter brockii brockii” International Journal of Biological Macromolecules 270 (2), 132404 (It was published as a part of Aycan Kayrav`s MSc thesis). DOI: 10.1016/j.ijbiomac.2024.132404

Hande Mumcu, Aycan Kayrav, Naciye Durmuş, Nevin Gul Karaguler (2023) “Cloning and characterization of thermostable amylopullulanase TbbApu and its C-terminal truncated variants with enhanced activity in organic solvents” Enzyme and Microbial Technology 164, 110176 (It was published as a part of Hande Mumcu`s MSc thesis). DOI: 10.1016/j.enzmictec.2022.110176